Glycohemoglobin A1c (HbA1c)
Glycohemoglobin A1c (HbA1c) Test Kit
In vitro test for the quantitative determination of HbA1c concentration in human whole blood on photometric systems. HbA1c is a product of hemoglobin (Hb) that produces a slow and continuous non-enzymatic glycation reaction under the action of high blood glucose. Glucose modifies hemoglobin specifically in its n-terminal valine residue to form glycated hemoglobin. Under normal physiological conditions, the production of non-enzymatic glycosylation reaction products is positively proportional to the concentration of reactants. Since hemoglobin concentration remains relatively stable, glycosylation levels are mainly dependent on glucose concentration and are also related to the length of hemoglobin and glucose exposure. Therefore, HbA1c is a good indicator for the average blood glucose level of patients at 2 to 3 months.
Under the action of protease, HbA1c is cut off from the n-terminal of its complement chain and releases glycosylated dipeptides. The Hb concentration can be obtained by measuring the absorbance of 500nm in the first reaction. In the second reaction, fructose peptide oxidase (FPOX) acts on glycosylated dipeptides to generate hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with chromogenic agents，and the concentration of HbA1c can be obtained by measuring the absorbance of 660nm. According to the obtained HbA1c concentration and Hb concentration, percentage composition of HbA1c was calculated.