Vaccinia Capping Enzyme
Catalog number: EN-1002
This product is the Vaccinia Capping Enzyme recombinantly expressed in E. coli.
Vaccinia Capping Enzyme can add the 7-methylguanosine cap (Cap0) to the 5’ terminus of mRNA. This structure can improve the stability of mRNA, and is indispensable to subsequent transport and translation.
Optimal reaction temperature: 37℃.
Definition of active unit: 1 active unit is defined as the amount of enzyme needed to incorporate 10 pmol of (α32P) GTP into 80nt transcript within 1h at 37℃.
Purity ≥ 95%, residual host cell DNA ≤ 100pg/mg, residual host cell protein ≤ 50ppm, residual endotoxin ≤10EU/mg, no residual RNase, endonuclease, exonuclease or protease, germ-free, pathogen-free.
Capping rate as high as 95%; the 5'-Cap structure effectively promoting the in vivo expression of mRNA.