Home » Glutamate Pyruvate Transaminase (ALT)

Glutamate Pyruvate Transaminase (ALT)

(Cat.NO.)HOEM0101

Production

Alanine Aminotransferase Kit (IFCC Method)

Application

ALT reagent is intended for quantitative determination of Alanine Aminotransferase activity in serum or plasma on photometric systems. Alanine aminotransferase (EC 2.6.1.2, ALT), formerly called Glutamic Pyruvic Transaminase (GPT), is one of liver-specific enzymes. It can catalyze the interconversion of amino acids and α-ketoacids by transfer of amino groups. It can catalyze the interconversion of amino acids and α-ketoacids by transfer of amino groups. Elevated ALT levels can indicate myocardial infarction, muscular dystrophy, especially in hepatobiliary diseases. Measurement of ALT is often used in diagnosis and monitoring treatment of liver diseases and heart diseases.

Principle

Alanine aminotransferase catalyzes the reversible transamination of L-alanine and α-oxoglutarate to pyruvate and L-glutamate. The pyruvate is then reduced to lactate in the presence of lactate dehydrogenase (LDH) with the concurrent oxidation of reduced β-nicotinamide adenine dinucleotide (NADH) to β-nicotinamide adenine dinucleotide (NAD). This change in absorbance is directly proportional to the activity of ALT in the sample.