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α-Amylase (AMY)

(Cat.NO.)HOEM2201

Production

α-Amylase Kit (IFCC Method)

Application

α-AMY reagent is intended for quantitative determination of α-Amylase (1, 4-α-D-glucanohydrolases) activity in serum, plasma or urine on photometric systems.α-Amylases originate from various organs and are mainly produced by the pancreas (P-type) and the salivary glands (S-type). α-Amylases catalyze the hydrolytic degradation of polymeric carbohydrates, such as amylose, amylopectin and glycogen by cleaving 1, 4-α-glucosidic bonds into various fragments. The pancreatic amylase is produced by the pancreas and released into the intestinal tract; the salivary amylase is synthesized in the salivary glands and secreted into saliva. Measurement of -amylase in serum and urine is mainly used for the diagnosis of pancreatic disorders, as well as for detecting the development of complications.

Principle

In the assay reaction, the substrate 4, 6- ethylidene-(G7)-1, 4-nitrophenyl-(G1) –α, D-maltoheptaoside (EPS-G7) is cleaved by α-amylases and subsequent hydrolysis of all the degradation products to p-nitrophenol with the aid of α-glucosidase (100% chromophore liberation). The increase in absorbance of the p-nitrophenol formed at 405 nm is directly proportional to the activity of α-amylases.