Glutamate Oxaloacetate Transaminase（AST）
Aspartate Aminotransferase Kit (IFCC Method)
AST reagent is intended for quantitative determination of AST activity in serum or plasma on photometric systems. Aspartate aminotransferase (EC 184.108.40.206, AST)，formerly called Glutamic Oxalacetic Transaminase (GOT), is present in both cytoplasm and mitochondria of cells，belonging to the transaminase family, which catalyze the conversion of amino acids and α-oxoglutarate by transfer of amino groups. AST is commonly found in various human tissues. The heart muscle is found to have the most activity of the enzyme, secondly in the brain, liver, gastric mucosa, skeletal muscle and kidneys. The serum AST present low activity in the healthy human body, but when these tissues injury or damage, AST is released into blood and results in high blood AST activity.
In the assay reaction, the AST catalyzes the reversible transamination of L-aspartate and α-oxoglutarate to oxaloacetate and L-glutamate. The oxaloacetate is then reduced to malate in the presence of malate dehydrogenase with NADH being oxidized to NAD+. The rate of the photometrically determined NADH decrease is directly proportional to the rate of formation of oxaloacetate and thus the AST activity.