Creatine Kinase-MB (CK-MB)
Creatine Kinase-MB Kit (IFCC Method)
In vitro test for the quantitative determination of creatine kinase-MB (CK-MB) activity in serum on photometric systems.Creatine kinase (CK) is an enzyme, which consists of isoenzymes mainly of the muscle (CK-M) and the brain (CK-B). CK exists in serum in dimeric form as CK-MM, CK-MB, and CK-BB and as macroenzyme. Determination of CK-MB values possesses highly specificity in myocardial damages. So, measurement of CK-MB is used for diagnosis and monitoring of myocardial infarction.
In the reaction, the creatine kinase-B catalyzes the transfer of a phosphate group from the creatine phosphate substrate to ADP. The subsequent formation of ATP is measured through the use of two coupled reactions catalyzed by hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH) which results in the production of the reduced form of NADPH from NADP. The rate of absorbency increase at 405nm is directly proportional to the activity of creatine kinase-B.