HiScript II 1st Strand cDNA Synthesis Kit
• Based on high-effective HiScript II Reverse Transcriptase: with high thermal stability ensuring the complex RNA secondary structure be denatured to obtain longer cDNA.
• A wide range of template input amount of total RNA (from 1 pg to 5 μg). Amplify cDNA fragments as long as 20 kb.
• Anchored Oligo(dT)23VN: further improves the specificity, efficiency and sensitivity of the first-strand cDNA synthesis.
• With different primer selection strategy, the first-strand cDNA products can be widely used for many applications, including cloning, hybridization, PCR amplification, and Real Time PCR.
The HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. The half-life of HiScript II at 50°C is > 240 min. Even at 55°C, the HiScript II can stay stable for a long time, which significantly benifits the transcription of RNA templates with complex secondary structures. In addition, the HiScript II has a improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (as long as 20 kb).
The HiScript II 1st Strand cDNA Synthesis Kit contains all the components necessary for the 1st strand cDNA synthesis. The products are suitable for PCR and qPCR. The 2× RT Mix contains an optimized buffer and dNTPs. The HiScript II Enzyme Mix contains the HiScript II Reverse Transcriptase and the RNase inhibitor. The Oligo-(dT)23VN has a better affinity to Ploy A+ RNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.
Note: The price is exc. VAT and shipping.
Brand: Vazyme Biotech.