M-MLV (H-) Reverse Transcriptase
• The RNase H activity was eliminated by mutagenesis to obtain cDNA with longer fragments.
• Stable and reliable reverse transcription for RNA templates with amount > 100 ng.
• The length of RT-PCR products is no longer than 5 kb.
• Suitable for 5’-RACE and cDNA library construction.
The wildtype Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase has the following activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, and RNase H. The M-MLV (H-) Reverse Transcriptase is a single-site mutant of M-MLV which contains no RNase H activity. Compared with M-MLV mutants obtained via deletion of the RNase H domain, this product, which retains a complete protein structure and polymerase activities, can be used for the synthesis of longer cDNA or the preparation of cDNA library.
Note: The price is exc. VAT and shipping.
Amount: 10,000 U
Brand: Vazyme Biotech.