Blood Direct PCR Kit V2

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Brand: Vazyme Biotech.

Blood Direct PCR Kit is designed to perform PCR directly from whole blood without DNA extraction or sample preparation. This kit can be used to perform PCR amplification from a wide range of templates including fresh blood, 4℃ stored blood, frozen blood, and dried blood stored on Whatman 903 ® or FTA ® commercial card. It is compatible with all conventional anticoagulants (EDTA, citrate, heparin, etc.).

This kit contains Phanta Blood Super-Fidelity DNA Polymerase, which is modified from Phanta Super-Fidelity DNA Polymerase. This enzyme displays superior resistance to the PCR inhibitors in the whole blood sample, and can amplify whole blood with concentration up to 40%. This kit can efficiently amplify genomic fragments of up to 10 kb from whole blood samples with the optimized 2 × Phanta Blood Buffer V2.

Phanta Blood Super-Fidelity DNA Polymerase produces blunt-ended products, which are applicable to ClonExpress cloning Kit (C112/C113). This kit supplies positive control primer mix which is compatible with mammalian and other vertebrate animals and can be used as positive control in experiments.